Recent Advances in Super-Resolution Fluorescence Imaging and Its Applications in Biology

作     者：Rongcheng Han Zhenghong Li Yanyan Fan Yuqiang Jiang 

作者机构：Institute of Genetics and Developmental BiologyChinese Academy of Sciences Institute of Opto-Electronic Materials and TechnologySouth China Normal University 

出 版 物：《Journal of Genetics and Genomics》 (遗传学报（英文版）)

年 卷 期：2013年第40卷第12期

页      面：583-595页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 1001[医学-基础医学(可授医学、理学学位)] 

基　　金：supported by the grants from the National Natural Science Foundation of China(Nos.11174089 and 61138003) the Instrument Developing Project of the Chinese Academy of Sciences(No.YZ201263) the Instrument Function Developing Project of the Chinese Academy of Sciences(No.yg2012032) the Key Project of Department of Education of Guangdong Province(No.cxzd1112) Guangzhou Municipal Science and Technology Program Project(No.2012J5100004) 

主　　题：Super-resolution Bio-imaging Fluorescence microscopy Optical diffraction limit 

摘      要：Fluorescence microscopy has become an essential tool for biological research because it can be minimally invasive, acquire data rapidly, and target molecules of interest with specific labeling strategies. However, the diffraction-limited spatial resolution, which is classically limited to about 200 nm in the lateral direction and about 500 nm in the axial direction, hampers its application to identify delicate details of subcellular structure. Extensive efforts have been made to break diffraction limit for obtaining high-resolution imaging of a biological specimen. Various methods capable of obtaining super-resolution images with a resolution of tens of nanometers are currently available. These super-resolution techniques can be generally divided into three primary classes： （1） patterned illumination- based super-resolution imaging, which employs spatially and temporally modulated illumination light to reconstruct sub-diffraction structures; （2） single-molecule localization-based super-resolution imaging, which localizes the profile center of each individual fluo- rophore at subdiffraction precision; （3） bleaching/blinking-based super-resolution imaging. These super-resolution techniques have been utilized in different biological fields and provide novel insights into several new aspects of life science. Given unique technical merits and commercial availability of super-resolution fluorescence microscope, increasing applications of this powerful technique in life science can be expected.