Breeding of High-Yield Salinomycin-Producing Streptomyces albus Strains by Low Energy N^+ Ion Beam Irradiation
Breeding of High-Yield Salinomycin-Producing Streptomyces albus Strains by Low Energy N^+ Ion Beam Irradiation作者机构：Beijing Radiation Center Heze Lukang Shelile Pharmaceutical Co.Ltd
出 版 物：《Agricultural Biotechnology》 (农业生物技术（英文版）)
年 卷 期：2012年第1卷第5期
页 面：55-56,F0003页
学科分类：081703[工学-生物化工] 08[工学] 0817[工学-化学工程与技术] 0836[工学-生物工程] 082203[工学-发酵工程] 0822[工学-轻工技术与工程]
主 题：Streptomyces albus N + ion beam irradiation Salinomycin Mutation breeding
摘 要：[Objective] This study aimed to explore the mutagenesis effects of N＋ ion beam implantation on Streptomyces a/bus and obtain high-yield salinomycin- producing mutant strain. [ Method ] Streptomyces a/bus strain S-11-04 was mutated with different doses of N ＋ implantation. The effects of low energy N ＊ implantation on the survival rate, colony morphology and salinomycin-producing ability were investigated. [ Result] The results showed that low energy N ＋ implantation can efficiently improve the positive mutation rate of Streptomyces albus; 13 mutant strains with high yield of salinomycin were isolated; to be specific, mutant strain N3- 6 has relatively good genetic stability with four continuous generations, and the titres of salinomycin were increased by 41% in the shake-flask culture and 20.5% in mass production compared with the control. [ Conclusion ] N ＋ ion beam irradiation is an effective method to obtain high-yield salinomycin-producing Streptomy- ces albus strain.
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