Detection　of　Catecholic　Compounds　with　Field-ampilfied　CZE-Amperometry

作     者：HU Shen, PANG Dai-wen , HU Yong-gang , CHENG Jie-ke (Department of Chemistry, Wuhan University, Wuhan, 430072 ) 

出 版 物：《Chemical Research in Chinese Universities》 (高等学校化学研究（英文版）)

年 卷 期：1997年第13卷第3期

页      面：221-228页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 

主　　题：Capillary zone electrophoresis Amperometric detection Stacking 

摘      要：On-column sample stacking technique has been used extensively in capillary zone electrophoresis (CZE) to enhance the sensitivity of the measurements with UV-Vis detection. However, the detection limits for CZE-separation are still limit- ed due to the poor sensitivity of a UV-Vis detector. We incorporated field-amplified sample stacking technique into CZE with amperometric detection. Compared with the conventional electromigration injection , a negative peak of water was observed and a great enhancement of signals was obtained. The results in phosphate buffer with sodium dodecyl sulfate (SDS ) and 2-N-(morpholino) ethane sulphonic acid ( MES ) buffer were compared. Subnanomolar concentration limits for cate- cholamine neurotransmitters (dopamine, norepinephrine , epinephriine) and isopro- terenol were achieved in the 25 um capillary with two or three orders of magnitude lower than those with amperometric detection reported previously.