Apoptosis of HeLa Cells Induced by Cisplatin and Its Mechanism

作     者：柳友清 邢辉 韩晓兵 石小燕 梁缝奇 陈刚 卢运萍 马丁 Youqing LIU,Hui XING,Xiaobing HAN,Xiaoyan SHI,Fengqi LIANG, Gang CHENG,Yunping LU,Ding MA Department of Obstetrics and Gynecology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China

作者机构：Department of Obstetrics and GynecologyTongji HospitalTongji Medical CollegeHuazhong University of Science and Technology Wuhan 430030China 

出 版 物：《Journal of Huazhong University of Science and Technology(Medical Sciences)》 (华中科技大学学报（医学英德文版）)

年 卷 期：2008年第28卷第2期

页      面：197-199页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：a grant of the National Re- search Project of China (Program 973) (No. 2002CB513100) 

主　　题：cell apoptosis cisplatin flow cytometry HPV E6 Bax Bcl-2 cervix cancer 

摘      要：To study the apoptosis induced by cisplatin in cervical cancer cell line HeLa and its mechanism, cell growth inhibition of cisplatin on HeLa cells was analyzed by MTT assay. Cell apoptosis was examined by cytometry and Hoechst33258 staining after treatment with cisplatin. The effects of cisplatin on transcription of E6 were analyzed by RT-PCR. The protein expressions of E6, p53, p21, Bax and Bcl-2 were studied by Western blotting. Cisplatin inhibited proliferation in a time- and dose-dependant manner. Cytometically, sub-G1 peak showed higher apoptosis rates in the experimental group than those in the control. Hoechst33258staining exhibited apoptosis induced by cisplatin. RT-PCR revealed that cisplatin decreased transcription of E6. Western blotting showed that cisplatin decreased protein expression of E6 and increased protein expression of p53, p21and Bax. It had no effect on protein expression of Bcl-2. It is concluded that cisplatin can induce apoptosis in HeLa cells by suppressing HPV E6 and thereby restoring the function of p53.