Effects of Short-Term Mechanical Stretch on the Proliferation and Osteogenic Differentiation of Mesenchymal Stem Cell and TRPC1 Expression

作     者：Yangbi Huang Yufan Zheng Shuwen Zhang Xinlan Chen Xiaoling Jia 

作者机构：Key Laboratory for Biomechanics and Mechanobiology of Ministry of EducationSchool of Biological Science and Medical EngineeringBeihang UniversityBeijing100083China Beijing Advanced Innovation Centre for BiomedicalEngineeringBeihang UniversityBeijing102402China 

出 版 物：《医用生物力学》 (Journal of Medical Biomechanics)

年 卷 期：2019年第34卷第A1期

页      面：66-67页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China ( 11872010) 

主　　题：mechanical stretch MSCs proliferation TRPC1 

摘      要：Objective Mechanical stretch regulates mesenchymal stem cell(MSC)function,which much more emphasis has been placed its prolonged effect on lineage differentiation,especially osteogenic *** contrast,there are few reports about its short term effect on MSC *** the present study,effects of short-term mechanical stretch on the proliferation and osteogenic differentiation of mesenchymal stem cell proliferation were *** addition,the stretchinfluenced expression of transient receptor potential cation channel,subfamily C,member 1(TRPC1)was also investigated due to its mechanosensitivity and positive correlation with MSC *** MSCs,harvested from rat bone marrow,were seeded on collagen l-coated silicone chamber and exposed to mechanical stretch with various magnitude(0%,5%,10%and 15%)or various duration(2 h,6 h,12 h and 24 h).Cell proliferation was examined by cell counting kit-8(CCK-8)assay and cell cycle *** gene and protein expression of two makers for osteogenic differentiation,collagen I and Cbfα1,and TRPC1 were determined by RT-PCR and western blotting,*** were harvested,and total RNA was isolated with Trizol reagent.A 2μg portion of total RNA was synthesized to cDNA according to the manufacturer s *** was used as a template for each PCR *** were solubilized in RIPA lysis buffer on ice for 30 *** sulfonyl fluoride(PMSF)was added to avoid *** portions of the cell lysates were separated on 10%sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE)and transferred to polyvinylidene fluoride(PVDF)*** membranes were incubated with primary antibodies to TRPC1 and GAPDH at4℃overnight to identify the specific *** PVDF membranes were washed with TBST three times and incubated with a horseradish peroxidase(HRP)-conjugated secondary *** bands were visualized using an enhanced chemiluminescent(ECL)