Effect of Biophysical Cues on Cell Reprogramming

作     者：Yang Song Song Li 

作者机构：Department of BioengineeringUniversity of California 

出 版 物：《医用生物力学》 (Journal of Medical Biomechanics)

年 卷 期：2019年第34卷第A1期

页      面：14-15页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 10[医学] 

基　　金：supported in part by a grant from the National Institute of Health ( HL121450) UCLA Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research Innovation Award 

主　　题：Effect Biophysical Cues Cell Reprogramming 

摘      要：Cell reprograming technologies have broad applications in cell therapy,disease modeling and drug *** reprogramming is the process of converting from one cell type into a very distantly related cell *** this direct conversion process,cells do not proceed through a pluripotent stage,which can be time-consuming and challenging due to spontaneous *** method also offers the advantage of circumventing the teratoma potential that is associated with using *** works have demonstrated that with the use of genetic manipulation,fibroblasts can be directly converted into other cell types,including neurons,cardiomyocytes,blood cell progenitors,and *** is well known that the microenvironment can directs cell fate,and in turn cells interact with or remodel their *** evidence suggests that biophysical factors such as the microtopography and mechanical property of cell adhesive substrates regulate a variety of cellular functions such as migration,proliferation and differentiation,which in turn can modulate wound healing,tissue remodeling and tumor growth,but there are limited number of studies on the roles of biophysical cues in cell reprogramming[1].Passive topographical cues offer a simple and effective method to improve reprogramming efficiency without the need for biochemical *** previous study has demonstrated that somatic cells cultured on the parallel microgrooves,which can replace the effects of small-molecule epigenetic modifiers and significantly improve the iPSCs reprogramming *** mechanism relies on the mechanomodulation of the cells’epigenetic state,specifically,an increase of histone H3 acetylation and H3K4 methylation[2].Additionally,in cardiomyocytes reprogramming study,culturing the fibroblasts on microgrooved substrate enhances the expression of cardiomyocyte genes by day 2 and improves the yield of partially reprogrammed cells at day *** combining microgrooved substra