Fluorescence polarization immunoassay for salinomycin based on monoclonal antibodies

作     者：Wang, Zhan Hui Cheng, Lin Li Shi, Wei Min Zhang, Su Xia Shen, Jian Zhong 

作者机构：National Reference Laboratories for Veterinary Drug Residue College of Veterinary Medicine China Agricultural University Beijing 100193 China 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：2010年第53卷第3期

页      面：552-554页

核心收录：

学科分类：07[理学] 0703[理学-化学] 

基　　金：support from the National Natural Science Foundation of China (Grant No 30830082) the Major State Basic Research Development Program of China (Grant No 2009CB118801) Beijing Excellent Doctoral Dissertation Fund (Grant No YB20081001902) 

主　　题：salinomycin tracer fluorescence polarization immunoassay monoclonal antibody 

摘      要：A fluorescence polarization immunoassay(FPIA) for the determination of salinomycin(SAL) was developed by using anti-SAL monoclonal antibodies(mAb).Fluorescein labeled SAL(tracer) was synthesized by the N-hydroxysuccinimide active ester method and purified using thin layer chromatography(TLC).The developed FPIA for SAL had a dynamic range from 0.60 to 2193 ng/mL with an IC50 value of 33.2 ng/mL and a detection limit(LOD) of 0.08 ng/*** significant cross-reactivities were observed with other drugs but 67.6% with narasin.