cpSSR: a New Tool to Analyze Chloroplast Genome of Citrus Somatic Hybrids

作     者：程运江 郭文武 邓秀新 

作者机构：华中农业大学作物遗传改良国家重点实验室武汉430070 

出 版 物：《Acta Botanica Sinica》 (Acta Botanica Sinica（植物学报：英文版）)

年 卷 期：2003年第45卷第8期

页      面：906-909页

核心收录：

学科分类：09[农学] 0902[农学-园艺学] 090201[农学-果树学] 

基　　金：国家自然科学基金(30123001) 国家高技术研究发展计划"863"项目(2001AA241141) 国际基金(IFS) 国际植物种质资源委员会(IPGRI) 

主　　题：Citrus, somatic hybrids microsatellite simple sequence repeat (SSR) chloroplast genome 

摘      要：Chloroplast simple sequence repeat (cpSSR) markers in Citrus were developed and successfully used to analyze chloroplast genome inheritance of Citrus somatic hybrids. Twenty-two previously reported cpSSR primer pairs from pine (Pinus thunbergii Parl.), rice (Otyza sativa L.) and tobacco (Nicotiana tabacum L.) were tested in Citrus, nine of which could amplify intensive PCR products by agarose gel electrophoresis. Chloroplast genome inheritance of Citrus somatic hybrids from nine fusions was then analyzed, and five of the nine pre-screened primer pairs showed polymorphisms by polyacrylamide gel electrophoresis. The results revealed the random inheritance nature of chloroplast genome in all analyzed Citrus somatic hybrids, which was in agreement with previous reports based on RFLP or CAPS analyses. It was also shown that cpSSR is a more efficient tool in chloroplast genome analyses of somatic hybrids in higher plants, compared with the conventional RFLP or CAPS analyses.