CCR3 monoclonal antibody inhibits airway eosinophilic inflammation and mucus overproduction in a mouse model of asthma

作     者：Hua-hao SHEN~2 Feng XU~2 Gen-sheng ZHANG Shao-bin WANG Wei-hua XU Department of Respiratory Diseases,the Second Affiliated Hospital,Zhejiang University College of Medicine,Hangzhou 310009,China 

作者机构：Department of Respiratory Diseases the Second Affiliated Hospital Zhejiang University College of Medicine Hangzhou China 

出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))

年 卷 期：2006年第27卷第12期

页      面：1594-1599页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：国家自然科学基金 浙江省自然科学基金 浙江省科技厅重点项目 

主　　题：asthma CCR3 monoclonal antibody eosinophilic inflammation mucus overproduction MUC5AC epidermal growth factor receptor 

摘      要：Aim:To explore the effect of a rat anti-mouse CC-chemokine receptor-3(CCR3)monoclonal antibody(CCR3 mAb)on airway eosinophilia and mucus overpro-duction in asthmatic ***:An asthma model was sensitized and chal-lenged by ovalbumin(OVA)in male C57BL/6 *** mice were givendual administration(intraperitoneal injection and aerosol inhalation)of CCR3 mAbor nonspecific rat IgG(ns-IgG).The number of total and differential inflammatorycells in the bronchial alveolar lavage fluid(BALF)was *** number,the goblet cell percentage(GCP)and airway mucus index(AMI)were measured inthe lung ***(IL)-5 levels in the BALF were *** expres-sion of MUC5AC and the epidermal growth factor receptor(EGFR)mRNA in thelung tissues was detected by semi-quantitative *** results were com-pared among the ***:CCR3 mAb significantly suppressed theincreased eosinophils in the BALF and lung tissues in OVA-challenged mice com-pared with ns-IgG-treated ***-5 levels in the BALF in CCR3 mAb and ns-IgGadministration mice exhibited no obvious changes relative to OVA-challengedasthmatic ***3 mAb reduced the increased GCP and AMI after OVA chal-lenge and decreased the enhanced expression of MUC5AC and EGFR mRNA inlung tissues in asthmatic ***:CCR3 mAb can significantlyinhibit airway eosinophilia and mucus overproduction in asthmatic ***-age of CCR3 may represent a new strategy to asthma therapy.