Relationship between acid phosphatase activity and P concentration in organs of Cyclopia and Aspalathus species,and a non-legume of the Cape Floristic Region

作     者：Sipho T.Maseko Felix D.Dakora 

作者机构：Department of Crop SciencesTshwane University of TechnologyPrivate Bag X680Pretoria 0001South Africa Chemistry DepartmentTshwane University of TechnologyPrivate Bag X680Pretoria 0001South Africa 

出 版 物：《Journal of Plant Ecology》 (植物生态学报（英文版）)

年 卷 期：2019年第12卷第2期

页      面：387-392页

核心收录：

学科分类：09[农学] 0903[农学-农业资源与环境] 090301[农学-土壤学] 

基　　金：The South African Research Chair in Agrochemurgy and PlantSymbioses, the National Research Foundation (No. 47720) theTshwane University of Technology are acknowledged for supportingthe research of F.D.D., and for providing a bursary to S.T.M. Weare grateful to the farmers (Mr Fritz Joubert and Mrs Rica Joubertat Koksrivier, and Mr Matie Taljaard and Mrs Erica Taljaard atKanetberg) for allowing us to collect plant and soil samples from theircommercial Honeybush tea plantations 

主　　题：Cape fynbos δ^(15)N Leucadendron strictum percent N derived from fixation P nutrition 

摘      要：Aims The role of tissue acid phosphatase(APase)activity of legumes and non-legumes in their P nutrition and adaptation to low-P soils is not well *** better understand this,a relationship between APase activ-ity and P concentration in leaves,stems,roots and nodules of legumes,Cyclopia and Aspalathus and a non-legume,Leucadendron strictum,all native to the P-poor soils of the Cape fynbos biome,was *** Plants were collected and each separated into leaves,stems and *** enzyme activity was assayed in soil using the p-nitrophenol method,while soil P and shoot P were measured using *** measure tissue APase activity,an acetate buffer was added into ground plant material and contents *** ace-tate buffer and a p-nitrophenyl solution were added to the superna-tant and contents *** incubation,NaOH(0.5 M)was added and absorbance read at 405 *** Findings At Koksrivier,Cyclopia genistoides exhibited the highest leaf enzyme activity whilst Aspalathus aspalathoides showed the highest enzyme activity in the *** both Kleinberg and Kanetberg,Cyclopia subternata and Cyclopia longifolia showed the highest APase activity in leaves,followed by stems and low-est in roots.P concentration closely mirrored enzyme activity in organs of all test species from each *** activity posi-tively correlated with P concentration in organs of all the test Cyclopia and Leucadendron species,indicating that intracellular APase activity is directly linked to P mobilization and transloca-tion in these *** of N derived from fixation was positively correlated with tissue APase activity in ***(r=0.911^(*)),***(r=0.868^(*))and Aspalathus cal-edonensis(r=0.957^(*)),suggesting that APase activity could be directly or indirectly linked to symbiotic functioning in these fynbos legumes,possibly via increased P supply to sites of N2 fixation.