Longitudinal directional movement of Alcian blue in Gephyrocharax Melanocheir fish: Revealing interstitial flow and related structure

作     者：Wei-bo ZHANG Xiao-jing SONG Ze WANG Guang-jun WANG Shu-yong JIA Yu-ying TIAN Hong-yan LI 张维波;宋晓晶;王泽;王广军;贾术永;田宇瑛;李宏彦

作者机构：Department of biomedical engineering Institute of Acupuncture &Moxibustion China Academy of Chinese Medical Sciences Beijing 100700 China Department of Endocrinology Guang'anmen Hospital China Academy of Chinese Medical Sciences Beijing 100053 China 

出 版 物：《World Journal of Acupuncture-Moxibustion》 (世界针灸杂志（英文版）)

年 卷 期：2019年第29卷第2期

页      面：127-132页

核心收录：

学科分类：10[医学] 

基　　金：Supported by the National Natural Science Foundation of China:81173206 

主　　题：Interstitial fluid Interstitial flow Meridians and collaterals Alcian blue Gephyrocharax Melanocheir fish 

摘      要：Background:Observing interstitial fluid(ISF)is very difficult because interstitial structure collapses and ISF disappears after tissue ***,ISF is colorless,and interstitial flow is weak in *** order to view the interstitial flow,special dye and animal model was chosen to explore the movement characteristic of interstitial flow and related ***:The Gephyrocharax Melanocheir(GM)fish,a special animal with translucent body,were placed into 0.03 g/L tricain solution for anesthesia.20-25μL Alcian blue(AB)solution which can stain acid mucopolysaccharide immobilized by the collagen net in connective tissue was injected into each fish at a single point with a rate of 2 μL/min via a micro-injection *** process of infusion and the movement of the AB in fish were record by a digital ***(fresh)frozen sections of AB tracks tissue were performed to observe the morphological ***:Several blue tracks were observed which were formed by longitudinal directional movements of AB *** back lateral track,the velocity and length of the movements were significantly fast and longer on the direction toward head than that toward tail(P0.01).For lateral middle track,the result was opposite,namely toward tail(P0.01).This phenomenon indicated an inherent ISF flow according to Darcy s *** study showed these tracks were just in septa composed by connective *** stained blue septa formed various shapes as interstitial space for ISF flow and connected with one another like a *** finding can help us to understand the essence of meridian in traditional Chinese medicine(TCM).Conclusions:The dynamic asymmetry of the AB tracks revealed interstitial flow in the GM fish *** implied an inherent interstitial flow along particular pathway formed by septa which may be a key to understand the nature and value of meridians and collaterals in health care.