Blend of Polyhydroxyalkanoates Synthesized By Lipase Positive Bacteria From Plant Oils

作     者：Nasir Javaid Rida Batool Nazia Jamil 

作者机构：Department of Microbiology and Molecular GeneticsUniversity of the PunjabQuaid-e-Azam CampusLahore-54590Pakistan 

出 版 物：《Journal of Renewable Materials》 (可再生材料杂志（英文）)

年 卷 期：2019年第7卷第5期

页      面：463-476页

核心收录：

学科分类：0202[经济学-应用经济学] 02[经济学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 020205[经济学-产业经济学] 0706[理学-大气科学] 0805[工学-材料科学与工程（可授工学、理学学位）] 

基　　金：Higher Education Commision  Pakistan  HEC  (NRPU-3443) 

主　　题：Bioplastics polyhydroxyalkanoates lipase positive bacteria plant oils,FT-IR 

摘      要：A total of 5 biochemically characterized lipase positive bacterial strains were screened for Polyhydroxyalkanoates(PHA)production by Nile blue staining and confirmation was done by Sudan Black *** production ability for all strains was optimized followed by time profiling calculation and comparison via using glucose and two plant oils i.e.,canola and mustard *** analysis showed that glucose can serve as a carbon source for maximum biomass(2.5 g/L CDW for strain 5)and PHA production(70.3%for strain 2).PHA produced by strain 2 was further analyzed for its chemical composition and type via Fourier Transform Infrared(FT-IR)*** revealed homopolymer(PHB)and copolymer(PHB-co-PHV)production of PHA(peaks at 1743 cm-1 and 2861 cm-1,respectively)with both canola and mustard oil unlike glucose which produced only homopolymer one i.e.,PHB(peaks at 1110 cm-1,1411 cm-1 and 1650 cm-1).Crystallinity of FT-IR analyzed PHA was calculated using mathematical formulas which showed decrease from glucose to canola to mustard *** study revealed that plant oils can serve as better carbon source to produce better quality(ductile and copolymer)***,16S rRNA gene sequencing analysis showed that strain 1,strain 2,strain 3,strain 4 and strain 5 are Stenotrophomonas sp.N3,Exiguobacterium sp.N4,Exiguobacterium ***3,Cellulosimicrobium sp.A8 and Klebsiella ***2,respectively.