Gene expression profiling of HepG_(2) cells after treatment with black tea polyphenols
作者机构：Scientific Research DepartmentHunan Province Institute of GerontologyHunan Provincial People’s Hospital/The First Affiliated Hospital of Hunan Normal UniversityChangshaChina Center of Reproductive MedicineHunan Provincial People’s Hospital/The First Affiliated Hospital of Hunan Normal UniversityChangshaChina Hunan Provincial Key Laboratory of Emergency and Critical Care MetabonomicsHunan Provincial People’s Hospital/The First Affiliated Hospital of Hunan Normal UniversityChangshaChina Pharmacy DepartmentTianmen First People’s HospitalTianmenHubeiChina
出 版 物：《BIOCELL》 (生物细胞（英文）)
年 卷 期：2018年第42卷第3期
页 面：99-104页
核心收录：
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：supported by grants from the Science and Technology Foundation of Health and Family Planning Commission of Hunan Province(No.C2019048) Science and Technology Bureau of Changsha(No.ZD1702024)
主 题：Black tea polyphenols Microarray HepG_(2)cells Gene expression
摘 要：This study aimed to determine the effects of black tea polyphenols on gene expression in hepatocellular cancer *** total RNA from HepG_(2) hepatocellular cancer cells treated with black tea polyphenols was subjected to Human 14K cDNA microarray ***-time PCR and Western blot analysis were conducted to verify microarray *** tea polyphenols treatment at the dose of 20 mg/L,40 mg/L or 80 mg/L for one to three days inhibited the growth of HepG_(2) cells in a dose and time dependent manner.A total of 48 genes showed more than two-fold change after black tea polyphenols treatment,including 17 upregulated genes and 31 downregulated genes,and they were involved in the regulation of cell growth,cell cycle,apoptosis,signaling,angiogenesis,tumor invasion and ***-time PCR analysis of the selected genes showed that their mRNA expression changes were consistent with the microarray *** addition,Western blot analysis of the selected genes showed that their protein expression changes were consistent with mRNA *** conclusion,gene expression profiles provide comprehensive molecular mechanisms by which black tea polyphenols exerts growth inhibition effects on cancer *** novel molecular targets identified in this study may be further exploited as therapeutic strategies for hepatocellular cancer.
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