CRISPR/Cas9-mediated ebony knockout results in puparium melanism in Spodoptera litura
作者机构：College of Life Sciences East China Normal University Shanghai China Key Laboratory of Insect Developmental and Evolutionary Biology Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of SciencesShanghai China Department of Biology University of Nevada Reno NV USA
出 版 物：《Insect Science》 (昆虫科学（英文版）)
年 卷 期：2019年第26卷第6期
页 面：1011-1019页
核心收录：
学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 0904[农学-植物保护] 0901[农学-作物学] 0713[理学-生态学]
基 金：国家自然科学基金 Strategic Priority Research Program of Chinese Academy of Sciences
主 题：CR1SPR/Cas9 ebony marker gene Spodoptera litura
摘 要：Insect body pigmentation and coloration are critical to adaption to the environment. To explore the mechanisms that drive pigmentation, we used the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) genome editing system to target the ebony gene in the non-model insect Spodoptera litura. Ebony is crucial to melanin synthesis in insects. By directly injecting Cas9 messenger RNA and ebony-specific guide RNAs into S. litura embryos, we successfully induced a typical ebony-deficient phenotype of deep coloration of the puparium and induction of melanin formation during the pupal stage. Polymerase chain reaction-based genotype analysis demonstrated that various mutations had occurred at the sites targeted in ebony. Our study clearly demonstrates the function of ebony in the puparium coloration and also provides a potentially useful marker gene for functional studies in S. litura as well as other lepidopteran pests.
维普期刊数据库