Recent advances in structural studies of the CRISPR-Cas-mediated genome editing tools

作     者：Yuwei Zhu Zhiwei Huang Yuwei Zhu;Zhiwei Huang

作者机构：HIT Center for Life Sciences School of Life Science and Technology Harbin Institute of Technology 

出 版 物：《National Science Review》 (国家科学评论(英文版))

年 卷 期：2019年第6卷第3期

页      面：438-451页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071007[理学-遗传学] 

基　　金：supported by the National Natural Science Foundation of China(31422014 31450001 and 21300605 to ZH) 

主　　题：CRISPR-Cas system adaptive immune viral infection genome editing 

摘      要：Clustered regularly interspaced short palindromic repeats(CRISPR) and accompanying CRISPR-associated(Cas) proteins provide RNA-guided adaptive immunity for prokaryotes to defend themselves against viruses. The CRISPR-Cas systems have attracted much attention in recent years for their power in aiding the development of genome editing tools. Based on the composition of the CRISPR RNA-effector complex, the CRISPR-Cas systems can be divided into two classes and six types. In this review, we summarize recent advances in the structural biology of the CRISPR-Cas-mediated genome editing tools, which helps us to understand the mechanism of how the guide RNAs assemble with diverse Cas proteins to cleave target nucleic acids.