The role and mechanism of 2deoxyglucose in reversing osimertinibacquired resistance of nonsmall cell lung cancer cell line

作     者：HAO Shuai 郝帅

作者机构：Dept Respir DisDaping HospArmy Milit Med UnivChongqing 400042 

出 版 物：《China Medical Abstracts(Internal Medicine)》 (中国医学文摘（内科学分册（英文版）)

年 卷 期：2019年第36卷第2期

页      面：98-98页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：NSCLC 2deoxyglucose lung cancer cell line 

摘      要：Objective To explore the role and mechanism of 2-deoxyglucose ( 2-dg) in reversing osimertinib-acquiredresistance of non-small cell lung cancer ( NSCLC) cellline. Methods The NSCLC line H1975 (purchased fromthe American Type Culture Collection) was conducted byinduction method in vitro to construct the osimertinib-resistanceNSCLC cell line H1975-OR. The osimertinib-resistanceof H1975-OR cell line was examined by 3-(4,5-Dimethylthiazol-2-yl )-2,5-diphenyltetrazolium bromide(MTT) assay,colony-formation assay,Ki67 incorporationassay and the expression of apoptosis-related *** glycolysis level was assayed by the lactic acidproduction measured in the culture medium supernatantof H1975 and H1975-OR. The expression of glycolysiskey enzymes (HK2,GLUT1,P-PKM2) and apoptosisrelatedprotein (BIM,Bcl-2) were detected by Westernblot.