Arabidopsis AGC protein kinases IREH1 and IRE3 control root skewing

作     者：Xiaozhen Yue Zhiai Guo Teng Shi Lizhen Song Youfa Cheng 

作者机构：Key Laboratory of Plant Molecular PhysiologyInstitute of BotanyChinese Academy of SciencesBeijing 100093China University of Chinese Academy of SciencesBeijing 100049China 

出 版 物：《Journal of Genetics and Genomics》 (遗传学报（英文版）)

年 卷 期：2019年第46卷第5期

页      面：259-267页

核心收录：

学科分类：0710[理学-生物学] 1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

基　　金：supported by the grants from the National Natural Science Foundation of China (31270330, 91217310, 91017008 and 31171389) the National Basic Research Program of China (2014CB943400) the National Key Research and Development Program of China (2016YFD0100402) the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA0801010402) the One-Hundred Talent Project of the Chinese Academy of Sciences to Y.C 

主　　题：AGC kinases IREH1 IRE3 Root skewing Microtubule Arabidopsis 

摘      要：AGC protein kinases play important roles in plant growth and development.Several AGC kinases in Arabidopsis have been functionally characterized.However,theAGC Othersubfamily,including IRE,IREH1,IRE3 and IRE4,has not been well understood.Here,we reported that ireh1 mutants displayed a root skewing phenotype,which can be enhanced by ire3 mutation.IREH1 and IRE3 were expressed in roots,consistent with their function in controlling root skewing.The fluorescence intensities of the microtubule marker KNpro:EGFP-MBD were decreased in ireh1,ire3 and ireh1 ire3 mutants compared to wild type.The microtubule arrangements in ireh1 and ireh1 ire3 mutants were also altered.IREH1 physically interacted with IRE3 in vitro and in planta.Thus,our findings demonstrate that IREH1 and IRE3 protein kinases play important roles in controlling root skewing,and maintaining microtubule network in Arabidopsis.