Study on the Interferation of the Concentration of Free Calcium in Peripheral Human Lymphocyte by Low Dose Penicillin, Using Fura-2 as Fluorescent Probe

作     者：Hai Yan WANG Dan Dan WANG Chun Gui ZHAO Ye Hong ZHOU Shao Min SHUANG Chuan DONG 

作者机构：School of Chemistry and Chemical Engineering Shanxi University Taiyuan 030006 Medical College of Datong University Datong 037000 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2006年第17卷第5期

页      面：665-666页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 10[医学] 

基　　金：This work was supported by the National Natural Science Foundation of China (No.20575038) the Foundation of Shanxi Provience (No.20031019) 

主　　题：Penicillin lymphocytes Fura-2 Ca^2+ ouabain. 

摘      要：The effect of penicillin on the human peripheral lymphocytes was studied by steady fluorescent technique and ratiometric fluorescence dye, Fura-2, The change of the free calcium concentration in cytosol was examined under different conditions. A characterization of Fura-2-Ca interaction in an isotonic saline solution showed that Ca^2＋ formed a 1：1 Fura-2-Ca complex with the apparent dissociation constant 1.81×10^-7 mol/L. The mechanism, by which penicillin induced the decrease of [Ca^2＋]i , was discussed in detail. The low dose of penicillin might modify the lymphocytes＇ immunology response by interfering the increase in the intracellular free calcium concentration.