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Flow-injection Chemiluminescence Determination of Trace Calf Thymus DNA

Flow-injection Chemiluminescence Determination of Trace Calf Thymus DNA

作     者:Min ZHOU, Yong Jun MA, Xiao Yong JIN, Xiu Lan TENG, Zi Yu ZHANG,Hui CHENInstitute of Chemistry, Northwest Normal University, Lanzhou 730070 

作者机构:Institute of Chemistry Northwest Normal University Lanzhou 730070 

出 版 物:《Chinese Chemical Letters》 (中国化学快报(英文版))

年 卷 期:2003年第14卷第10期

页      面:1051-1053页

核心收录:

学科分类:081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学] 

基  金:国家自然科学基金 

主  题:Chemiluminescence flow-injection calf thymus DNA imidazole rhodamine B. 

摘      要:A flow injection procedure for the determination of calf thymus DMA (CT DNA) over the range 2.1x10-6;.1x10-1 B.g mL-1 is described, based on measurement of the enhanced chemiluminescence emission of rhodamine B-Ce(IV) system, activated by imidazole-HCl buffer solution. This method is highly sensitive, with the linearity range broadened to five orders of magnitude. It has been applied to determine CT DNA in synthetic sample with satisfactory results.

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