A cell-based high-throughput approach to identify inhibitors of influenza A virus

作     者：Qian Gao Zhen Wang Zhenlong Liu Xiaoyu Li Yongxin Zhang Zhizhen Zhang Shan Cen 

作者机构：Institute of Medicinal BiotechnologyChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijingChina Department of BiochemistryGuangdong Medical CollegeZhanjiang524023China 

出 版 物：《Acta Pharmaceutica Sinica B》 (药学学报（英文版）)

年 卷 期：2014年第4卷第4期

页      面：301-306页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：This work was supported in part by National S&T Major Special Project on Major New Drug Innovation(No.2012ZX09301-002-004)(S.C.) National Science and Technology Major Project,“China Mega-Project for Infectious Disease”(No.2013ZX 10004601-002),and the Xiehe Scholar(S.C.) 

主　　题：Cell-based assay Gaussia luciferase Influenza A virus High-throughput screen 

摘      要：Influenza is one of the most common infections threatening public health worldwide and is caused by the influenza *** emergence of drug resistance has led to an urgent need to develop new anti-influenza *** this study we established a 293T cell line that constitutively synthesizes a virus-based negative strand RNA,which expresses Gaussia luciferase upon influenza A virus *** this cell line,an assay was developed and optimized to search for inhibitors of influenza virus *** studies and statistical analyses presented herein demonstrate the sensitivity and reproducibility of the assay in a high-throughput format(Z 0 factor value40.8).A pilot screening provides further evidence for validation of the *** together,this work provides a simple,convenient,and reliable HTS assay to identify compounds with anti-influenza activity.