A cell-based high-throughput approach to identify inhibitors of influenza A virus
识别流行性感冒的禁止者的一条基于房间的高产量的途径病毒 <sup></sup><sup></sup>作者机构:Institute of Medicinal BiotechnologyChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijingChina Department of BiochemistryGuangdong Medical CollegeZhanjiang524023China
出 版 物:《Acta Pharmaceutica Sinica B》 (药学学报(英文版))
年 卷 期:2014年第4卷第4期
页 面:301-306页
核心收录:
学科分类:1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金:This work was supported in part by National S&T Major Special Project on Major New Drug Innovation(No.2012ZX09301-002-004)(S.C.) National Science and Technology Major Project,“China Mega-Project for Infectious Disease”(No.2013ZX 10004601-002),and the Xiehe Scholar(S.C.)
主 题:Cell-based assay Gaussia luciferase Influenza A virus High-throughput screen
摘 要:Influenza is one of the most common infections threatening public health worldwide and is caused by the influenza *** emergence of drug resistance has led to an urgent need to develop new anti-influenza *** this study we established a 293T cell line that constitutively synthesizes a virus-based negative strand RNA,which expresses Gaussia luciferase upon influenza A virus *** this cell line,an assay was developed and optimized to search for inhibitors of influenza virus *** studies and statistical analyses presented herein demonstrate the sensitivity and reproducibility of the assay in a high-throughput format(Z 0 factor value40.8).A pilot screening provides further evidence for validation of the *** together,this work provides a simple,convenient,and reliable HTS assay to identify compounds with anti-influenza activity.