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A cell-based high-throughput approach to identify inhibitors of influenza A virus

识别流行性感冒的禁止者的一条基于房间的高产量的途径病毒 <sup></sup><sup></sup>

作     者:Qian Gao Zhen Wang Zhenlong Liu Xiaoyu Li Yongxin Zhang Zhizhen Zhang Shan Cen 

作者机构:Institute of Medicinal BiotechnologyChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijingChina Department of BiochemistryGuangdong Medical CollegeZhanjiang524023China 

出 版 物:《Acta Pharmaceutica Sinica B》 (药学学报(英文版))

年 卷 期:2014年第4卷第4期

页      面:301-306页

核心收录:

学科分类:1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基  金:This work was supported in part by National S&T Major Special Project on Major New Drug Innovation(No.2012ZX09301-002-004)(S.C.) National Science and Technology Major Project,“China Mega-Project for Infectious Disease”(No.2013ZX 10004601-002),and the Xiehe Scholar(S.C.) 

主  题:Cell-based assay Gaussia luciferase Influenza A virus High-throughput screen 

摘      要:Influenza is one of the most common infections threatening public health worldwide and is caused by the influenza *** emergence of drug resistance has led to an urgent need to develop new anti-influenza *** this study we established a 293T cell line that constitutively synthesizes a virus-based negative strand RNA,which expresses Gaussia luciferase upon influenza A virus *** this cell line,an assay was developed and optimized to search for inhibitors of influenza virus *** studies and statistical analyses presented herein demonstrate the sensitivity and reproducibility of the assay in a high-throughput format(Z 0 factor value40.8).A pilot screening provides further evidence for validation of the *** together,this work provides a simple,convenient,and reliable HTS assay to identify compounds with anti-influenza activity.

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