SKIP Confers Osmotic Tolerance during Salt Stress by Controlling Alternative Gene Splicing in Arabidopsis
SKIP Confers Osmotic Tolerance during Salt Stress by Controlling Alternative Gene Splicing in Arabidopsis作者机构:College of Life Sciences Hebei Normal University Shijiazhuang Hebei 050021 China College of Life Sciences Capital Normal University Beijing 100048 China College of Life Sciences Peking University Beijing 100871 China These authors contributed equally to this article.
出 版 物:《Molecular Plant》 (分子植物(英文版))
年 卷 期:2015年第8卷第7期
页 面:1038-1052页
核心收录:
学科分类:0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种]
基 金:国家自然科学基金 Beijing Municipal Government Science Foundation supported by grants from the National Basic Research Program (973 Program) of the Ministry of Science and Technology of China
主 题:salt response osmotic tolerance SKIP alternative splicing posttranscriptional regulation
摘 要:Deciphering the mechanisms underlying plant responses to abiotic stress is key for improving plant stress resistance. Much is known about the regulation of gene expression in response to salt stress at the tran- scriptional level; however, little is known about this process at the posttranscriptional level. Recently, we demonstrated that SKIP is a component of spliceosome that interacts with clock gene pre-mRNAs and is essential for regulating their alternative splicing and mRNA maturation. In this study, we found that skip-1 plants are hypersensitive to both salt and osmotic stresses, and that SKIP is required for the alter- native splicing and mRNA maturation of several salt-tolerance genes, including NHXl, CBL1, P5CS1, RCl2A, and PATIO. A genome-wide analysis revealed that SKIP mediates the alternative splicing of many genes under salt-stress conditions, and that most of the alternative splicing events in skip-1 involve intron retention and can generate a premature termination codon in the transcribed mRNA. SKIP also controls alternative splicing by modulating the recognition or cleavage of 5' and 3' splice donor and acceptor sites under salt-stress conditions. Therefore, this study addresses the fundamental question of how the mRNA splicing machinery in plants contributes to salt-stress responses at the posttranscriptional level, and provides a link between alternative splicing and salt tolerance.