Antiproliferative and apoptosis-inducing potential of 3β-hydroxy-△5-steroidal congeners purified from the soft coral Dendronephthya putteri

作     者：Thilina U. JAYAWARDENA Won Woo LEE I. P. Shanura FERNANDO K. K. Asanka SANJEEWA Lei WANG Tee Gee LEE Young Jin PARK Chang-ik KO You-Jin JEON 

作者机构：Department of Marine Life Sciences Jeju National University Jeju 690-756 Republic of Korea Department of Hotel Cooking Nutrition Jeonnam Provincial College Jeollanam-do Republic of Korea Department of Family Medicine College of Medicine Dong-A University Busan 602-715 Republic of Korea Choung Ryong Fisheries Co. Ltd. 7825 Iljudong-ro Namwon-eup Seogwipo-si Jeju-do Republic of Korea 

出 版 物：《Journal of Oceanology and Limnology》 (海洋湖沼学报（英文）)

年 卷 期：2019年第37卷第4期

页      面：1382-1392页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 0908[农学-水产] 0707[理学-海洋科学] 0815[工学-水利工程] 10[医学] 

基　　金：Supported by the "Regional Specialized Industry Development Program",Ministry of Trade,Industry,and Energy(MOTIE),Korea supervised by the Korea Institute for Advancement of Technology(KIAT) 

主　　题：Dendronephthya putteri soft coral antiproliferative agent HL-60 MCF-7 apoptosis steroidal congeners 

摘      要：The exploration and identification of antiproliferative phytochemicals have received increased attention in medicinal chemistry. In particular, research focused on the toxicology of marine natural products has increased in recent years. Terpenoids, among many secondary metabolites, have been demonstrated to act as effective anticancer agents. Soft corals, a group of marine invertebrates, produce a variety of terpenoids with biofunctional properties. The current study presents the extraction, purification, and identification of sterol congeners from the soft coral Dendronephthya putteri. The method involves 50% chloroform-methanol extraction, polar column fractionation, and analysis through GC-MSn. Dose-dependent antiproliferative activity was observed within the sterol-rich fraction (DPCMH 2-4), which consisted of 3β-hydroxy-Δ5-steroidal congeners. This fraction inhibited the growth of HL-60 and MCF-7 cells with IC50 values of 25.27±1.43 and 22.81±0.15 μg/mL, respectively. Apoptotic body formation, DNA damage, cell cycle arrest, and apoptotic cell signaling pathway activation were also observed, reinforcing the dose-dependent antiproliferative and apoptosis-inducing activity of 3β-hydroxy-Δ5-steroidal congeners. To our knowledge, this is the first report of anticancer agent identification from the soft coral D. putteri. Based on the observations, these steroidal congeners are promising candidates for the development of anticancer drugs.