Morphology and ontogeny of directly differentiating shoot buds and somatic embryos in Santalum album L.

作     者：Rakhshanda Akhtar Anwar Shahzad 

作者机构：Plant Biotechnology SectionDepartment of BotanyAligarh Muslim University 

出 版 物：《Journal of Forestry Research》 (林业研究（英文版）)

年 卷 期：2019年第30卷第4期

页      面：1179-1189页

核心收录：

学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 0907[农学-林学] 0829[工学-林业工程] 09[农学] 0901[农学-作物学] 

基　　金：The financial assistance for conducting this study was provided by Maulana Azad National Fellowship-SRF(Award number MANF-2013-14-MUS–BIH-21399),UGC,India For the purchase of chemicals,provided by projects with Vide numbers SB/YS/LS-156/2013 and SB/FT/LS-364/2012 

主　　题：Direct somatic embryos Emblings Histology Hypocotyl Ontogenic 

摘      要：Santalum album *** a commercially important tree that yields essential oil of high medicinal *** research through organogenesis and embryogenesis has been documented but no report depicts comparative ontogeny of directly differentiating shoot buds(SB)and somatic embryos(SE).In the present study aseptic seedling derived hypocotyl segments(HC)and hypocotyl+root junction(HC+R)were used to induce SBs and SEs,*** differences between the structures were confirmed using scanning electron microscopy and histological *** medium containing 6-benzyladenine or BA(2.5μM)produced highest number of direct SB,while MS+BA(7.5μM)proved suitable for higher frequency of SE *** differentiating structures attained growth when transferred to MS medium containing a combination of BA and anaphthalene acetic acid or silver nitrate(AgNO3).A combination of indole-3-butyric acid and silver nitrate(AgNO3)in half-strength woody plant medium and lesser osmotic concentration(2%sucrose),induced rhizogenesis.