Effect of siRNAs on HSV-1 Plaque Formation and Relative Expression Levels of RR mRNA

作     者：Zhe Ren Shen Li Qiao-li Wang Yang-fei Xiang Yun-xia Cui Yi-fei Wang Ren-bin Qi Da-xiang Lu Shu-min Zhang Pei-zhuo Zhang 

作者机构：Biomedicine research and development center of Jinan University Guangdong Guangzhou 510632 China Medical College of Jinan University Guangdong Guangzhou 510632 China National Engineering Research Center of Genetic Medicine Guangdong Guangzhou 510632 China National Institute for the Control of Pharmaceutical and Biological Products Beijing 100050China Shanghai GenePharma Co. Ltd Shanghai 201203 China 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2011年第26卷第1期

页      面：40-46页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：The Nation "863" Program of China(2006AA02A226) The Joint Funds of National Science Foundation of China (U0632010) The State KeyLaboratory of Phytochemistry and Plant Resources in West China Chinese Academy of Sciences (O807B11211, O807E21211) "211 grant of MOE" 

主　　题：Small interfering RNA (siRNA) Herpes simplex virus type 1 (HSV-1) Ribonucleotide reductase(RR) Gene 

摘      要：RNA interference (RNAi) is a process by which introduced small interfering RNA (siRNA) can cause the specific degradation of mRNA with identical sequences. The human herpes simplex virus type 1 (HSV-1) RR is composed of two distinct homodimeric subunits encoded by UL39 and UL40,respectively. In this study,we applied siRNAs targeting the UL39 and UL40 genes of HSV-1. We showed that synthetic siRNA silenced effectively and specifically UL39 and UL40 mRNA expression and inhibited HSV-1 replication. Our work offers new possibilities for RNAi as a genetic tool for inhibition of HSV-1 replication.