Construction of Suppression Subtractive Hybridization Library for Testis of Male Tilapia Under the Stress of Methomyl

作     者：Shunlong MENG Tao LIU Chao SONG Cong ZHANG Liping QIU Jiazhang CHEN Pao XU 

作者机构：Freshwater Fisheries Research Center of Chinese Academy of Fishery SciencesKey Open Laboratory of Inland Fishery Ecological Environment and Resources of Chinese Academy of Fishery ScienceWuxi 214081China Wuxi Fishery College of Nanjing Agricultural UniversityWuxi 214081China 

出 版 物：《Agricultural Biotechnology》 (农业生物技术（英文版）)

年 卷 期：2019年第8卷第3期

页      面：73-77页

学科分类：09[农学] 

基　　金：Supported by Basic Scientific Research Fund for Chinese Academy of Fishery Sciences(2015C02XK01) Youth Natural Scientific Foundation of Jiansu Province(BK20150117) Earmarked Fund for China Agriculture Research System(CARS-46) 

主　　题：Methomyl Tilapia Suppression subtractive hybridization cDNA library Testis 

摘      要：[Objectives]This study was conducted to construct forward and reserve libraries of suppression subtractive hybridization (SSH) in the testis of male tilapia under the stress of methomyl by using SSH technology.[Methods]Using male tilapia as the test animal,the forward and reserve libraries of SSH in the testis of tilapia under the stress of methomyl were constructed by using the SSH technology.[Results]45 expressed sequence tags (ESTs) were obtained,and 25 expressed sequence tags were successfully noted,including 13 forward libraries and 12 reverse *** genes with confirmed functions were classified into five *** related to catalytic activity and cell characteristics were up-regulated,while genes related to structural molecule s activity and biological process were *** expression amount of integrin β1 was up-regulated,while serine/threonine protein kinase pim-3,Ca^2+-ATPase,Na^+-K^+-ATPase and ribosomal protein L22 were down-regulated.[Conclusions]The research results could lay a foundation for revealing the molecular mechanism of methomyl s reproductive toxicity to tilapia.