Micro-Raman spectroscopy of single leukemic cells

作     者：蔡长美 陈荣 林居强 李永增 冯尚源 

作者机构：Key Laboratory of Optoelectronic Science and Technology for Medicine Ministry of Education of ChinaFujian Provincial Key Laboratory for Photonics TechnologyFujian Normal University 

出 版 物：《Chinese Optics Letters》 (中国光学快报（英文版）)

年 卷 期：2008年第6卷第12期

页      面：938-940页

核心收录：

学科分类：1002[医学-临床医学] 0808[工学-电气工程] 07[理学] 0809[工学-电子科学与技术（可授工学、理学学位）] 070302[理学-分析化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 100214[医学-肿瘤学] 0703[理学-化学] 10[医学] 0702[理学-物理学] 

基　　金：supported by the National Natural Science Foundation of China(No.60778046,60711120031) the Natural Science Foundation of Fujian Province(No.A0740010,2007J0318) 

主　　题：Cell culture Infrared devices Laser excitation Nucleic acids Principal component analysis Raman scattering Raman spectroscopy Spectrum analysis 

摘      要：The Raman spectra from leukemic cell line （HL60） and normal human peripheral blood mononuclear cells （PBMCs） are obtained by confocal micro-Raman spectroscopy using near-infrared laser （785 nm） excitation. The scanning range is from 500 to 2000 cm^-1. The two average Raman spectra of normal PBMCs and carcinoma cells have clear differences because their structure and amount of nucleic acid, protein, and other major molecules are changed. The spectra are also compared and analyzed by principal component analysis （PCA） to demonstrate the two distinct clusters of normal and transformed cells. The sensitivity of this technique for identifying transformed cells is 100%.