Determination of enantiomeric impurity of etomidate by high performance liquid chromatography

作     者：Xiao-Dan Wang,Xiao-Juan Chai,Su Zeng Department of Pharmaceutical Analysis and Drug Metabolism,College of Pharmaceutical Sciences,Zhejiang University,Hangzhou 310058,China. 

作者机构：Department of Pharmaceutical Analysis and Drug Metabolism College of Pharmaceutical Sciences Zhejiang University Hangzhou 310058 China 

出 版 物：《Journal of Pharmaceutical Analysis》 (药物分析学报（英文版）)

年 卷 期：2010年第22卷第2期

页      面：102-104页

学科分类：1007[医学-药学(可授医学、理学学位)] 10[医学] 

基　　金：supported by the National Key Project of China (2009ZX09304-003) 

主　　题：etomidate enantiomeric impurity high performance liquid chromatography 

摘      要：Objective To determine enantiomeric impurity of etomidate using high performance liquid chromatography. Methods (R)-etomidate and (S)-etomidate were separated on a CHIRALPAK AD-H column. The mobile phase consisted of 20∶80(v/v) isopropanol-n-hexane. The flow rate of the mobile phase was 0.5mL/min. The detected wavelength was 242nm. Results (R)-etomidate and (S)-etomidate could be separated completely under these conditions. The precision of (R)-etomidate was 1.57% (n=3). The limit of detection of (R)-etomidate was 4.25ng/mL. The average percentage content of (S)-etomidate was 0.09% in the samples. Conclusion The method was repeatable and sufficiently sensitive to determine the enantiomeric impurity of etomidate. It allows the quantitation of the impurities at the 0.085% (w/w) level relative to etomidate at a concentration of the test solution of 5mg/mL.