Identification and expression profiles analysis of odorant-binding proteins in soybean aphid,Aphis glycines(Hemiptera:Aphididae)

作     者：Ling Wang Ying-Dong Bi Ming Liu Wei Li Miao Liu Shu-Feng Di Shuai Yang Chao Fan Lei Bai Yong-Cai Lai Ling Wang;Ying-Dong Bi;Ming Liu;Wei Li;Miao Liu;Shu-Feng Di;Shuai Yang;Chao Fan;Lei Bai;Yong-Cai Lai

作者机构：Institute of Crop Cultivation and TillageHeilongjiang Academy of Agricultural SciencesHarbinChina Heilongjiang Academy of Agricultural Sciences Postdoctoral ProgramHarbinChina Virus-free Seedling Research InstituteHeilongjiang Academy of Agricultural SciencesHarbinChina College of AgricultureNortheast Agricultural UniversityHarbinChina 

出 版 物：《Insect Science》 (昆虫科学（英文版）)

年 卷 期：2020年第27卷第5期

页      面：1019-1030页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 

基　　金：We gratefully acknowledge Dr.Tao Zhong(Shenyang Academy of Agricultural Sciences,China)for his con-structive reviews of the manuscript.This、work was supported by the Natural Science Foundation of Hei-longjiang(C2018060) Heilongjiang Postdoctoral Fund(LBH-Z16187) Scientific Research Project of Hei-longjiang Academy of Agricultural Sciences(2017ZC10,2017SJ032 and 2018JJPY004) National Key Research and Development Program(2017YFE0111000),National Natural Science Foundation ofChina(31771823) Ma-jor Project of Research and Development of Applied Tech-nology in Heilongjiang Province(GA18B101) 

主　　题：Aphis glycines expression profiles fluorescence binding assay odorant-binding proteins 

摘      要：The soybean aphid,Aphis glycines,is an extreme specialist and an important invasive pest that relies on olfaction for behaviors such as feeding,mating,and ***-binding proteins(OBPs)play a vital role in olfaction by binding to volatile compounds and by regulating insect sensing of the *** this work we used rapid amplification of complementary DNA ends technology to identify and characterize 10 genes encoding *** OBPs(AglyOBPs)belonging to 3 subfamilies,including 4 classic OBPs,5 Plus-C OBPs,and one Minus-C *** real-time polymerase chain reaction demonstrated variable specific expression patterns for the 10 genes based on developmental stage and aphid tssue *** levels of 7 AglyOBPs(2,3,4,5,7,9,and 10)were highest in the 4th instar,indicating that the 4th nymphal instar is an important developmental period during which soybean aphids regulate feeding and search for host ***-specific expression results demonstrated that AglyOBP2,7,and 9 exhibited significantly higher expression levels in ***,ligand-binding analysis of5 OBPs demonstrated binding of AglyOBP2 and AglyOBP3 to a broad spectrum of volatiles released by green leaf plants,with bias toward 6-to 8-carbon chain volatiles and strong binding of AglyOBP7 to *** together,our findings build a foundation of knowledge for use in the study of molecular olfaction mechanisms and prov ide insights to guide future soybean aphid research.