Inactivation of infectious bursal disease virus by binary ethylenimine and formalin

作     者：HABIB M. HUSSAIN I. FANG W.H. RAJPUT Z.I. YANG Z.Z. IRSHAD H. 

作者机构：Department of Veterinary Microbiology University of Agriculture Faisalabad 38040 Pakistan） Institute of Preventive Veterinary Medicine Zhejiang University Hangzhou 310029 China Department of Livestock and Fisheries Animal Husbandry Sindh Hyderabad 71000 Pakistan 

出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)

年 卷 期：2006年第7卷第4期

页      面：320-323页

核心收录：

学科分类：090601[农学-基础兽医学] 09[农学] 0906[农学-兽医学] 

基　　金：Project (No. PSF/Res/P-AU/Bio (246)) supported by Pakistan Sci-ence Foundation (PSF) 

主　　题：Infectious bursal disease virus (IBDV) Binary ethylenimine (BEI) Inactivation 

摘      要：In this experiment conducted to study the inactivation dynamics of infectious bursal disease virus （IBDV） by binary ethylenimine （BEI） in comparison with formalin, IBDV was isolated from the bursa of infected chickens and its confirmation was done by agar gel precipitation test. Viral suspensions were subjected to inactivation with BEI and formalin for pre-set time in- tervals. BEI was employed at concentrations of 0.001 and 0.002 mol/L while formalin was used at 0.1% and 0.2%. Sampling was done at 6, 12, 24, 36 and 48 h of incubation and samples were tested for their inactivation status in 9-day-old embryonated eggs and 3-week-old broiler chickens. IBDV was completely inactivated by 0.001 and 0.002 mol/L BEI after 36 h of incubation at 37℃, whereas formalin at 0. 1% and 0.2% concentrations inactivated IBDV in 24 h.