Hermetia illucens larvae as a potential dietary protein source altered the microbiota and modulated mucosal immune status in the colon of finishing pigs

作     者：Miao Yu Zhenming Li Weidong Chen Ting Rong Gang Wang Xianyong Ma 

作者机构：Institute of Animal ScienceGuangdong Academy of Agricultural SciencesGuangzhou 510640GuangdongPeople’s Republic of China State Key Laboratory of Livestock and Poultry BreedingGuangzhou 510640GuangdongPeople’s Republic of China Key Laboratory of Animal Nutrition and Feed Science in South ChinaMinistry of AgricultureGuangdong Public Laboratory of Animal Breeding and NutritionGuangzhou 510640GuangdongPeople’s Republic of China Guangdong Engineering Technology Research Center of animal Meat quality and Safety Control and EvaluationGuangzhou 510640GuangdongPeople’s Republic of China 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2019年第10卷第4期

页      面：990-1004页

核心收录：

学科分类：090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 09[农学] 

基　　金：supported by the Presidential Foundation of the Guangdong Academy of Agricultural Sciences(201802B,201621) Guangdong Modern Agro-industry Technology Research System(2016LM1080,2017LM1080) 

主　　题：Colon Finishing pigs Hermetia illucens larvae Immune status Microbial metabolites Microbiota 

摘      要：Background: Insects, such as Hermetia illucens larvae, are rich in chitin and proteins, and represent a suitable feed ingredient replacement for animals. However, little is known about the effect of administering H. illucens larvae on intestinal microbiota, bacterial metabolite profiles, and mucosal immune status in animals. This study aimed to investigate the effects of administering H. illucens larvae on colonic microbiota and bacterial metabolites production in finishing pigs. Seventy-two crossbred(Duroc × Landrace × Large White) female pigs(initial body weight, 76.0 ± 0.52 kg) were randomly allocated to three different dietary treatments: a control diet(Control group) and two diets corresponding to 4%(H1 group) and 8%(H2 group) H. illucens larvae inclusion levels, respectively. Each treatment consisted of eight pens(replicates), with three pigs per pen. After 46 days of feeding, eight pigs per treatment(n =8) were slaughtered, and the colonic digesta and mucosa were collected for microbial composition and microbial fermentation products, and genes expression ***: The results showed that the H1 diet significantly increased the abundance of Lactobacillus,Pseudobutyrivibrio, Roseburia, and Faecalibacterium compared with those in the control group(P 0.05), with a decrease in the abundance of Streptococcus. The numbers of Lactobacillus, Roseburia, and Clostridium cluster XIVa were significantly greater in the H1 group than in the control group(P 0.05). Meanwhile, H2 diet increased the number of Clostridium cluster XIVa compared with the control group(P 0.05). For colonic metabolites, total short chain fatty acids, butyrate, and isobutyrate concentrations were significantly higher in the H1 group than those in the control group(P 0.05);the H1 treatment caused a striking decrease in protein fermentation compared with the control group, as the concentrations of total amines, cadaverine, tryptamine, phenol, p-cresol, and skatole were significantly lo