4-HNE Induces Apoptosis of Human Retinal Pigment Epithelial Cells by Modifying HSP70

作     者：Lei-lei YANG Hao CHEN Jun WANG Ting XIA Hong SUN Chun-hui YUAN Shi-liang LIU Jian-bin CHEN 

作者机构：Department of Ophthalmology Wuhan Fourth Hospital (Puai Hospital) Huazhong University of Science and Technology Wuhan 430030China Department of PathologyZhongnan Hospital of Wuhan University Wuhan 430070China Department of Laboratory Medicine Wuhan Medical and Health Center for Women and Children Huazhong University of Science and Technology Wuhan 430016 China Department of Ophthalmology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China 

出 版 物：《Current Medical Science》 (当代医学科学（英文）)

年 卷 期：2019年第39卷第3期

页      面：442-448页

核心收录：

学科分类：0710[理学-生物学] 1001[医学-基础医学(可授医学、理学学位)] 0703[理学-化学] 10[医学] 

基　　金：This project was supported by grants from the Natural Science Foundation of Hubei Province, China (No.2009CDB115,and No.2012FKB02444) Natural Science Foundation of Health and Family Planning Commission of Wuhan Municipality (No.WX18Q03,and No.WX18Q27) 

主　　题：4-hydroxy-2-nonenal HSP70 XIAP human retinal pigment epithelial cells apoptosis 

摘      要：The role of heat shock protein 70 (HSP70) in apoptosis of human retinal pigment epithelial cells (ARPE-19) induced by 4-hydroxy-2-nonenal (4-HNE) was explored. Different concentrations of 4-HNE were used to stimulate ARPE-19 cells, and apoptosis was measured by flow cytometry. The expression of apoptotic-related proteins, HSP70, X-linked inhibitorof- apoptosis (XIAP), Bcl-2, and Bax were quantified by Western blotting. HSP70 and XIAP overexpression plasmids, or their corresponding siRNAs were transfected into ARPE-19 cells using Lipofectamine. 2000. Co-immunoprecipitation and Western blotting were used to detect the effect of 4-HNE on the expression of HSP70 and the binding level between 4-HNE and HSP70. The results showed that 4-HNE induced late apoptosis in ARPE-19 cells, accompanied by elevated levels of 4-HNE-modified IISP70, but it did not affect HSP70 protein expression. 4-HNE-modified HSP70 down-regulated the expression of the apoptosis inhibitory protein XIAP. Overexpression of HSP70 or XIAP inhibited 4-HNE-induced apoptosis of ARPE-19 cells. It was suggested that 4-HNE could promote XIAP degradation by modification of HSP70 to induce late apoptosis of human retinal pigment epithelial cells.