A Novel Ratiometric Fluorescent Probe for Highly Sensitive and Selective Detection of β-Galactosidase in Living Cells

作     者：Min Chen Lixuan Mu Xingxing Cao Guangwei She Wensheng Shi 

作者机构：Key Laboratory of Photochemical Conversion and Optoelectronic MaterialsTechnical Institute of Physics and ChemistryChinese Academy of SciencesBeijing 100190China University of Chinese Academy of SciencesBeijing 100049China 

出 版 物：《Chinese Journal of Chemistry》 (中国化学（英文版）)

年 卷 期：2019年第37卷第4期

页      面：330-336页

核心收录：

学科分类：08[工学] 0817[工学-化学工程与技术] 0703[理学-化学] 

基　　金：National Key R&D Program of China(2016YFA0200800) NSFC(51672284) Beijing Natural Science Foundation(2181002) Chinese Academy of Sciences(1A1111KYSB20180017,QYZDJ-SSW-JSC032,XDB17000000). 

主　　题：has be 

摘      要：β-Galactosidase,a glycoside hydrolase enzyme,has been proved to be an important biomarker of cell senescence and primary ovarian cancer.Effective detection of β-galactosidase has attracted wide attention.Herein,one ratiometric fluorescent probe has been successfully synthesized for detecting the β-galactosidase in living cells.The as-prepared probe exhibits two emission peaks at 490 nm and 530 nm,respectively,and the ratio of fluorescence intensities from the two emission peaks could be utilized to monitor the β-galactosidase.This present ratiometric fluorescent probe is,therefore,very promising for effective,sensitive,and selective detection of the β-galactosidase in living cells.