The Inhibitory Effect of Curcumin on Ornithine Decarboxylase against Hepatic Carcinoma
The Inhibitory Effect of Curcumin on Ornithine Decarboxylase against Hepatic Carcinoma作者机构：Biochemistry Division Chemistry Department Faculty of Science Tanta University Tanta Egypt Head Researcher of Special Food and Nutrition Department Food Technology Research Institute Agricultural Research Center Giza Egypt
出 版 物：《Journal of Biosciences and Medicines》 (生物科学与医学（英文）)
年 卷 期：2019年第7卷第5期
页 面：127-145页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：Curcumin-HepG2 Cells-Apoptosis Ornithine Decarboxylase Bax Cell Cycle
摘 要：Curcumin the active component of turmeric is widely used as an anticancer agent for treating many human cancers. This study aimed at the extraction of curcumin from Curcuma Longa and investigates its therapeutic effect as ornithine decarboxylase (ODC) inhibitor in HepG2 cells. The proliferation of HepG2 cells was carried out by using the MTT assay. In addition, cell cycle analysis was evaluated by using the flow-cytometric technique. Our results showed that curcumin has the ability to inhibit the proliferation of HepG2 cells with IC50 of 24.79 μg/ml and induced G2/M cell cycle arrest. Moreover, it caused an elevation in the intracellular concentration of Ca2+. Moreover, in the curcumin administration the downregulation expression level of ODC and Bcl-2 genes (p ≤ 0.05) was significant found. On the other hand, upregulation in the expression level of P53, Bax, and caspase-3 genes (p ≤ 0.05). This study concluded that curcumin may be considered as a new saving candidate for the future progress of antitumor agents.
维普期刊数据库