Improvement of Growth and Periplocin Yield of Periploca sepium Adventitious Root Cultures by Altering Nitrogen Source Supply

作     者：ZHANG Jian GAO Wen-yuan WANG Juan LI Xing-lin XIAO Pei-gen 

作者机构：School of Traditional Chinese Materia MedicaTianjin University of Traditional Chinese MedicineTianj in 300193China 

出 版 物：《Chinese Herbal Medicines》 (中草药（英文版）)

年 卷 期：2011年第3卷第3期

页      面：-页

学科分类：090603[农学-临床兽医学] 09[农学] 0906[农学-兽医学] 

基　　金：MaiN PrOject Of TiaNjiN ScieNce aNd TechNOlOgy SuppOrt PrOgram (09ZCKFSH01100) 

主　　题：adventitious root nitrogen source Periploca sepium periplocin yield two-stage culture 

摘      要：Objective To increase the ultimate yield of periplocin in Periploca sepium adventitious root cultures by a two-stage culture based on nitrogen *** Firstly,the effects of nitrogen source(NH4+-NO3-) at different ratios and different total initial nitrogen amounts on the accumulation of biomass and secondary metabolites in adventitious root cultures of *** were investigated,and growth and production media for the two-stage culture based on the above results were *** The highest biomass and periplocin content were obtained in the culture medium of 15 mmol/L total nitrogen amount with NH4+-NO3-(1:2) and 30 mmol/L total nitrogen amount with nitrate as the sole nitrogen *** adopting a fed-batch cultivation strategy,the dry weight adventitious root,periplocin content and yield were increased by 136%,108%,and 389%,respectively when compared with those of the control,reaching up to 8.13 g/L,157.15 μg/g,and 1277.63 μg/L,***,it was found that in the process of two-stage culture,the adventitious roots grew thicker significantly after they were transferred into production medium *** The ultimate yield of periplocin in *** adventitious root cultures could be significantly increased by a two-stage culture based on nitrogen source.