Leukocyte cell-derived chemotaxin 2 inhibits development of atherosclerosis in mice

作     者：Wen-Ming He Ting Dai Jiong Chen Jian-An Wang 

作者机构：Department of Cardiology of the Second Affiliated HospitalZhejiang University School of MedicineHangzhou Zhejiang 310009China Department of Cardiologythe Affiliated Hospital of Medical School of Ningbo UniversityNingbo Zhejiang 315010China Laboratory of Biochemistry and Molecular BiologySchool of Marine SciencesMeishan CampusNingbo UniversityNingbo Zhejiang 315832China 

出 版 物：《Zoological Research》 (动物学研究（英文）)

年 卷 期：2019年第40卷第4期

页      面：317-323页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 07[理学] 09[农学] 

基　　金：supported by the Program for the National Natural Science Foundation of China(31772876) Ningbo Municipal Bureau of Science and Technology(2018A610389) Scientific Innovation Team Project of Ningbo(2015C110018) K.C.Wong Magna Fund in Ningbo University 

主　　题：Leukocyte cell-derived chemotaxin 2(LECT2) Atherosclerosis Inflammation Lipid metabolism 

摘      要：Leukocyte cell-derived chemotaxin 2 (LECT2), a multifunctional hepatokine, is involved in many pathological conditi ons. However, its role in atherosclerosis remains undefined. In this study, we admimistered vehicle or LECT2 to male Apoe^-/- mice fed a Western diet for 15 weeks. Atherosclerotic lesions were visualized and quantified with Oil-red O and hematoxylin staining. The mRNA expression levels of MCP-1, MMP-1, IL-8 IL-1β, and TNF-a were analyzed by quantitative real-time polymerase chain reaction. Serum TNF-a, IL-1β, IL-8, MCP-1, and MMP-1 concentrations were measured by en zyme-li nked immuno sorbent assay. CD68, CD31, and a-SMA, markers of macrophages, endothelial cells, and smooth muscle cells, respectively, were detected by immuno staining. Results showed that LECT2 reduced total cholesterol and low-density lipoprotein concentrations in serum and inhibited the development of atherosclerotic lesions, accompanied by reductions in inflammatory cytokines and lower MCP-1, MMP-1, TNF-a, IL-8, and IL-1β mRNA abundanee. Furthermore, LECT2 decreased CD68, but in creased cr SMA in atherosclerotic lesi ons, suggesting an in crease in smooth muscle cells and reduction in macrophages. In summary, LECT2 inhibited the development of atherosclerosis in mice, accompanied by reduced serum total cholesterol concentration and lower inflammatory responses.