Photoacoustic nanoprobe forβ-galactosidase activity detection and imaging in vivo

作     者：Qin Zeng Yunxia Wu Tao Zhang 

作者机构：MOE Key Laboratory of Laser Life Science&Institute of Laser Life Science College of BiophotonicsSouth China Normal University Guangzhou 510631P.R.China 

出 版 物：《Journal of Innovative Optical Health Sciences》 (创新光学健康科学杂志（英文）)

年 卷 期：2019年第12卷第3期

页      面：16-23页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 

基　　金：This research is supported by the National Natural Science Foundation of China(21771065) the Natural Science Foundation of Guangdong Province,China(2017A020215088) Pearl River Nova Program of Guangzhou,Guangdong Province,China(201806010189) The Scientific and Technological Planning Project of Guangzhou,Guangdong Province,China(201805010002) 

主　　题：β-galactosidase photoacoustic imaging 

摘      要：Detection and visualization ofβ-galactosidase(β-gal)is essential to reffect its physiological and pathological effects on human health and disease,but it is still challenging to precisely trackβ-gal in vivo owing to the limitation of current analytical *** our work,we reported a photoacoustic(PA)nanoprobe for selective imaging of the endogenousβ-gal in *** nanoprobe Cy7-β-gal-LP was constructed by encapsulation of a near-infra red(NIR)dye Cy7-β-gal within a liposome(LP,DSPE-PEG2000-COOH).The dye Cy7-β-gal was synthesized based on a dye Cy-OH where the hydroxyl group was replaced by aβ-D-galactopyranoside residue,which can be recognized byβ-gal as an enzyme hydrolytic *** the addition ofβ-gal,the absorbance of Cy7-β-gal exhibited a significant red shift with the absorption peak moved from 600 nm to 680 nm,which should generate a switch-on PA signal at 680 nm in the presence ofβ-*** addition,as theffuorescence of the dye was totally quenched due to aggregation within the liposome,Cy7-β-gal-LP exhibited high PA conversion *** the nanoprobe,we achieved the selective PA detection and imaging ofβ-gal in the tumor-bearing mice.