Purification of RhIFN- in Bacteria Bodies with Acetic Acid-Water as Mobile Phase in RPLC

作     者：Ye Hua SHEN Qi Dong ZHANG Xin Du GENG Zhi Qing ZHANG Yun De HOU Institute of Modern Separation Science. Shaanxi Provincial Key Laboratory of Modern Separation Scicllce, Northwest University. Xi ’an 710069 Institute of Virus, Chinese Academy of Peventi 

作者机构：Institute Modern Separation Science Shaanxi Provincial Key Laboratory M. Northwest University Xi'an 710069 China Institute of Virus Chinese Academy Preventive Medicine Beijing 100052 China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2000年第11卷第9期

页      面：799-802页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 

基　　金：This work was supported by the National Natural Science Foundation of China!(29675017 39880003) 

主　　题：reversed-phase liquid chromatography recombinant human interferon- purification acetic acid mobile phase 

摘      要：Thr extract of E. containing recombinant human interferon- (rhIFN-) with 7.0 mol/L guanidine hydrochloride (Gu . HCl) was directly injected into a column of reverse phase liquid chromatography (RPLC) to separate and purify rhIFN- with acetic acid-water as mobile phase. Gu I-ICI and most impure proteins can be separated by this way. Compared with the usual dilution method, the bioactivity recovery of the purified rhIFN- was found to be over 500%. In addition, compared to common organic solvents employed ill RPLC, acetic acid has higher freezing point, and therefore, it is easy to concentrate the aim-protein by freeze-drying when acetic acid-water is used as mobile phase ill RPLC.