Celastrus orbiculatus Extracts Inhibit Human Hepatocellular Carcinoma Growth by Targeting mTOR Signaling Pathways

作     者：QIAN Ya-yun LI Wen-yuan YAN Yan ZHAO Xue-yu YANG Ting FANG Chuan-ci HOU Jing-jing LIU Yan-qing 

作者机构：Institute of Traditional Chinese Medicine and Western MedicineSchool of MedicineYangzhou UniversityYangzhou (225009)Jiangsu ProvinceChina Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile DiseasesYangzhou (225001)Jiangsu ProvinceChina Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and ZoonosesYangzhou (225009)Jiangsu ProvinceChina 

出 版 物：《Chinese Journal of Integrative Medicine》 (中国结合医学杂志（英文版）)

年 卷 期：2019年第25卷第11期

页      面：845-852页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1005[医学-中医学] 1002[医学-临床医学] 10[医学] 

基　　金：Supported by the National Natural Science Foundation of China(No.81403232) the National Natural Science Foundation of Jiangsu Province(No.BK20171290,BK2012686) the Doctoral Fund of the Ministry of Education of China(No.20133250120003) 

主　　题：Celastrus orbiculatus hepatocellular carcinoma mammalian target of rapamycin apoptosis 

摘      要：Objective:To characterize the molecular mechanism underlying the antineoplastic activity of Celastrus orbiculatus ***(COE).Methods:The human hepatocellular carcinoma HepG2 cells with mammalian target of rapamycin(mTOR)knockdown expressed(HepG2/mTOR-)were constructed using molecular biological *** vitro,the HepG2/mTOR-cells were treated with COE at various concentrations(10,20,40,80,160 and 320μg/mL).Cell viability was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)*** to the half-maximal inhibitory concentration(IC50)value(140 mg/L),the concentrations of COE in the subsequent experiment was set to alleviate *** HepG2/mTOR-cells were divided into 5 groups:negative control(untreated),COE treatment groups(40,80,120 mg/L COE)and positive control group(cisplatin,DDP,2 mg/L),***-type HepG2 cells were used as a blank *** effects of COE on the cell apoptosis were analyzed by flow cytometry and transmission electronic microscopy(TEM),*** protein expression levels of mTOR signal pathways were determined by Western *** vivo,HepG2/mTOR-cells(2×106 cell/mice)were subcutaneously injected into the right flank of nude ***-six female nude mice were randomly assigned to 6 groups according to body weight(6 mice per group)as follows:solvent vehicle control,Banmao Capsule treated group(BM,195 mg/kg),Tegafur,Gimeracil and Oteracil Potassium Capsules(10 mg/kg)treated group,and different dosages of COE(10,20,40 mg/kg)*** growth was monitored and immunohistochemical staining was used to examine the expression of apoptosis-related proteins in tumor ***:COE inhibited the proliferation significantly in a concentration-dependent manner in HepG2/mTOR-cells(P0.01).COE significantly induced the apoptosis of HepG2/mTOR-cells(P0.01),and the apoptotic bodies can be observed under *** significantly inhibits the proteins expression of mTORrelate