Gonadotrophin-releasing hormone-Ⅰ and -Ⅱ stimulate steroidogenesis in prepubertal murine Leydig cells in vitro

作     者：Yung-Ming Lin Ming-Yie Liu Song-Ling Poon Sew-Fen Leu Bu-Miin Huang 

作者机构：Department of Urology College of Medicine National Cheng Kung University Tainan 70101 Taiwan China Department of Environmental and Occupational Health College of Medicine National Cheng Kung University Tainan 70101 Taiwan China Department of Cell Biology and Anatomy College of Medicine National Cheng Kung University Tainan 70101 Taiwan China Institute of Bioindustrial Technology College of Human Ecology HungKuang University Taichung 43302 Taiwan China 

出 版 物：《Asian Journal of Andrology》 (亚洲男性学杂志（英文版）)

年 卷 期：2008年第10卷第6期

页      面：929-936页

核心收录：

学科分类：1006[医学-中西医结合] 1002[医学-临床医学] 100602[医学-中西医结合临床] 10[医学] 

主　　题：gonadotrophin-releasing hormone Leydig cells murine steroidogenesis stimulation 

摘      要：Aim： To study the effect and mechanism of gonadotrophin-releasing hormone （GnRH） on murine Leydig cell steroidogenesis. Methods： Purified murine Leydig cells were treated with GnRH-Ⅰ and -Ⅱ agonists, and testosterone production and steroidogenic enzyme expressions were determined. Results： GnRH-Ⅰ and -Ⅱ agonists significantly stimulated murine Leydig cell steroidogenesis 60%-80% in a dose- and time-dependent manner （P 〈 0.05）. The mRNA expressions of steroidogenic acute regulatory （STAR） protein, P450scc, 3β-hydroxysteroid dehydrogenase （HSD）, but not 17β-hydroxylase or 17β-HSD, were significantly stimulated by both GnRH agonists with a 1.5- to 3-fold increase （P 〈 0.05）. However, only 3β-HSD protein expression was induced by both GnRH agonists, with a 1.6- to 2-fold increase （P 〈 0.05）. Conclusion： GnRH directly stimulated murine Leydig cell steroidogenesis by activating 3β-HSD enzyme expression.