In vitro anti-inflammatory effects of different solution fractions of ethanol extract from Melilotus suaveolens Ledeb

作     者：ZHANG Xiao-yu TAO Jun-yan ZHAO Lei HUANG Zhi-jun XIONG Fu-liang ZHANG Shu-ling LI Chong-ming XIAO Fei 

作者机构：College of Life Science and Technology Huazhong University of Science and Technology Wuhan 430074 China Department of Hepatology and Infectious Disease Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 China Hubei Engineering Technology Center of Modernization Traditional Chinese Medicine Wuhan 430054 China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2007年第120卷第22期

页      面：1992-1998页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 10[医学] 

主　　题：Melilotus suaveolens Ledeb high performance liquid chromatography fingerprint anti-inflammatory RAW 264. 7 cells 

摘      要：Background Melilotus suaveolens Ledeb （M. suaveolens Ledeb） has long been used as a folk medicine in inflammation-related therapy. This study was undertaken to determine the anti-inflammatory effect of the plant. Methods Petroleum ether fraction, ethyl acetate fraction, n-butanol fraction, aqueous fraction were obtained from ethanol extract of M. suaveolens Ledeb and evaluated by high performance liquid chromatography （HPLC）. While dexamethasone （DM） was used as a positive control, the effects of different solution fractions of ethanol extract on tumor necrosis factor α （TNF-α） mRNA, cyclooxygenase 2 （COX-2） mRNA, COX-2 and nuclear factor KB （NF-KB） of LPS-stimulated RAW 264.7 cells were studied by real-time PCR, Westem blot analysis and immunocytochemical assay, respectively. Results Coumarin was one of the main ingredients in different solution fractions of ethanol extract except the aqueous fraction with no inflammatory effect. The petroleum ether fraction, ethyl acetate fraction and n-butanol fraction of ethanol extract could inhibit the production of TNF-α mRNA, COX-2 mRNA and NF-KB to some extent. Conclusions Different solution fractions of ethanol extract from M. suaveolens Ledeb had similar anti-inflammatory effect as did dexamethasone except the aqueous fraction. Coumarin was likely to be essential to the anti-inflammatory effect, and other ingredients might attribute to their different anti-inflammatory effects from the HPLC fingerprint.