Discovery of a Homolog of Siderophilin in a Plant
Discovery of a Homolog of Siderophilin in a Plant作者机构：Institute of Genetics and Developmental Biology the Chinese Academy of Sciences Beijing 100101 China
出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)
年 卷 期：2005年第47卷第12期
页 面：1409-1411页
核心收录：
学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学]
主 题：antibacterial iron-binding domain lactoferrin siderophilin transferrin.
摘 要：Members belonging to the siderophilin family are iron-binding and iron-transporting proteins, which includes transferrin and lactoferrin. They have only been found in animals previously. If siderophilin could be found in and isolated from a plant, its production and subsequent extensive application could be increased. The present study is the first to report the discovery of a homolog of siderophilin in a plant. In order to purify antifreeze proteins from Ammopiptanthus mongolicus （Maxim.） Cheng f., the authors processed the proteins from the leaves using techniques such as column chromatography using DEAE-Cellulose-52, gel filtration via Sephacryl S-100 HR medium, hydrophobic interaction chromatography, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Mass spectroscopy was performed on the three proteins purified and the sequence of one of the proteins （containing 32 amino acids） was found to have 97% homology with the corresponding part of one type of human lactoferrin. Moreover, one of the two peptides belongs to an iron-binding domain. So, it is possible that siderophilin also exists in plants and plays a role as an antibacterial and antifungal, among other actions.
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