Amlodipine inhibits matrix metalloproteinases expression and secretion in mouse macrophage

作     者：Yamin CAO Shiwen WANG Haiyun WU Yamin CAO, Shiwen WANG, Haiyun WU Institute of Geriatric Cardiology, General Hospital of Chinese PLA, Beijing 100853, China

作者机构：Institute of Geriatric Cardiology General Hospital of Chinese PLA Beijing 100853 China Institute of Geriatric Cardiology General Hospital of Chinese PLA Beijing 100853 China Institute of Geriatric Cardiology General Hospital of Chinese PLA Beijing 100853 China 

出 版 物：《Journal of Geriatric Cardiology》 (老年心脏病学杂志（英文版）)

年 卷 期：2005年第2卷第4期

页      面：240-242页

核心收录：

学科分类：10[医学] 

主　　题：amlodipine macrophage matrix metalloproteinase reverse transcriptase polymerase chain reaction gelatin zymography. 

摘      要：Objective To investigate whether the calcium channel blocker amlodipine could inhibit macrophage matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) expression and secretion. Methods Peritoneal macrophages were isolated from BALB/C mice and incubated with low (5μg/L ), middle (15μg/L) and high (305μg/L) concentrations of amlodipine, or in the medium alone (controls) for 24 hours, and the expression and secretion of MMP-2 and MM-9 of the cells were analyzed by RT-PCR and gelatin zymography. Results Compared with controls, amlodipine at low concentration had no significant effects on the expression and secretion of either MMP-2 and MMP-9 (P0.05) at middle concentrationit it could inhibited MMP-2 and MMP-9 expressions completely and significantly reduced the secretion of MMP-9 (P0.05); but it had no effect on the secretion of MMP-2. At high concentration it also inhibited MMP-2 and MMP-9 expression completely. Conclusion Amlodipine at 15 ig/L inhibited the expression of MMP-2 and MMP-9 and reduced the secretion of MMP-9, suggesting that amlodipine may stabilize atherosclerotic plaque.