Altered microRNA profiles of testicular biopsies from patients with nonobstructive azoospermia
作者机构：Department of UrologyPeking University Third HospitalBeijing 100191China Department of AndrologyPeking University Third HospitalBeijing 100191China Department of Human Sperm BankPeking University Third HospitalBeijing 100191China Department of Reproductive Medicine CenterPeking University Third HospitalBeijing 100191China
出 版 物：《Asian Journal of Andrology》 (亚洲男性学杂志（英文版）)
年 卷 期：2020年第22卷第1期
页 面：100-105页
核心收录：
学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学]
基 金：supported by the Peking University Clinical + X project Youth Fund 国家自然科学基金 北京市自然科学基金
主 题：male infertility microRNA nonobstructive azoospermia spermatogenesis
摘 要：Many studies have shown that microRNAs(miRNAs)play vital roles during the ***,little is known about the altered miRNA profiles of testicular tissues in nonobstructive azoospermia(NOA).Using microarray technology,the miRNA expression profiles of testicular biopsies from patients with NOA and of normal testicular tissues were *** analyses were conducted to predict the enriched biological processes and functions of identified *** microarray data were validated by quantitative reverse transcriptase polymerase chain reaction(qRT-PCR),the results of which were then validated with a larger sample *** between the miRNA expression levels and clinical characteristics were *** operating characteristic(ROC)curve analysis was used to evaluate the diagnostic ability of miRNAs for *** clustering showed that 129 miRNAs were significantly differentially expressed between the NOA and control *** analysis indicated that the differentially expressed miRNAs were involved in spermatogenesis,cell cycle,and mitotic *** the subsequent qRT-PCR assays,the selected miRNA expression levels were consistent with the microarray results,and similar validated results were obtained with a larger sample *** clinical characteristics were significantly associated with the expression of certain *** particular,we identified a combination of two miRNAs(miR-10b-3p and miR-34b-5p)that could serve as a predictive biomarker of *** study provides altered miRNA profiles of testicular biopsies from NOA patients and examines the roles of miRNAs in *** profiles may be useful for predicting and diagnosing the presence of testicular sperm in individuals with azoospermia.
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