菌寄生真菌纤细齿梗孢一丝氨酸-苏氨酸蛋白激酶基因OPK1的克隆及序列分析
Cloning and characterizing of the gene OPK1 encoding a serine-threonine protein kinase in mycoparasite fungi Olpitrichum tenellum作者机构:山东农业大学植物保护学院泰安271018 聊城大学植物保护系聊城252000
出 版 物:《植物病理学报》 (Acta Phytopathologica Sinica)
年 卷 期:2010年第40卷第3期
页 面:329-332页
核心收录:
学科分类:090601[农学-基础兽医学] 09[农学] 0906[农学-兽医学]
基 金:国家自然科学基金(30571246) 山东省教育厅项目(J08LF56)
主 题:蛋白激酶基因 菌寄生真菌 苏氨酸 丝氨酸 序列分析 有丝分裂原活化蛋白激酶 细胞内信号转导 可逆磷酸化
摘 要:Using degenerate PCR and TAIL-PCR,a protein kinase gene OPK1(Genebank accession No.:EU417815) was cloned from mycoparasite fungi Olpitrichum ***1 has an open reading frame of 2 031 bp interrupted by two introns(108 bp,84 bp) and putatively encodes a protein of 676 *** analysis indicated that OPK1 was most similar to other serine-threonine protein kinase in *** blot analysis indicated that OPK1 is present as a single copy in the ***-PCR showed it could be transcribed both in the phase of spore germination and hyphal growth.