Influence of Single Base Change in Shine-Dalgarno Sequence on the Stability of *** Plasmid PSM604
Influence of Single Base Change in Shine-Dalgarno Sequence on the Stability of *** Plasmid PSM604作者机构：Department of Pediatrics Tongji Hospital Tongji Medical University Wuhan Department of Molecular Biology The Immunobiological Research Center of Chiron-Biocine Siena Italy
出 版 物：《Journal of Huazhong University of Science and Technology(Medical Sciences)》 (华中科技大学学报（医学英德文版）)
年 卷 期：2000年第20卷第3期
页 面：183-185页
学科分类：1001[医学-基础医学(可授医学、理学学位)] 10[医学]
主 题：B. Subtilis site directed mutagenesis Shine Dalgarno sequence subtilisin
摘 要：*** expression plasmids generally require a stringent Shine Dalgarno Sequence(SDS). Site directed mutagenesis was explored to change the Shine Dalgarno Sequence from AAAAATGGGG (mutant type) to AAAAAGGGGG (wild type) in recombinant plasmid PSM604. The single base substitution made the plasmid with wild SDS unstable in structure and segregation. The interaction of SDS with subtilisin leader sequence of PSM604 might be responsible for the instability of plasmid.
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