Thermo-sensitive Poly(DEGMMA-co-MEA) Microgels: Synthesis, Characterization and Interfacial Interaction with Adsorbed Protein Layer

作     者：Zhen-bing Li Yan-hui Xiang Xian-jing Zhou Jing-jing Nie Mao Peng 杜滨阳 

作者机构：MOE Key Laboratory of Macromolecular Synthesis and Functionalization Department of Polymer Science & EngineeringZhejiang University Department of Chemistry Zhejiang University 

出 版 物：《Chinese Journal of Polymer Science》 (高分子科学（英文版）)

年 卷 期：2015年第33卷第11期

页      面：1516-1526页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 0817[工学-化学工程与技术] 070304[理学-物理化学(含∶化学物理)] 08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 

基　　金：financially supported by the National Natural Science Foundation of China(Nos.21274129 and 21322406) the Fundamental Research Funds for the Central Universities(No.2014XZZX003-21) the third level of 2013 Zhejiang Province 151 Talent Project Open Research Fund of State Key Laboratory of Polymer Physics and Chemistry Changchun Institute of Applied Chemistry,Chinese Academy of Sciences 

主　　题：QCM Poly(DEGMMA co MEA) microgels Thermosensitive Proteins Interfacial interaction 

摘      要：The novel microgels, poly[di（ethylene glycol） methyl ether methacrylate-co-2-methoxyethyl acrylate] poly（DEGMMA-co-MEA） microgels, were synthesized. The poly（DEGMMA-co-MEA） microgels were thermo-sensitive and exhibited a volume phase transitive temperature（VPTT） of 14–22 ℃. The incorporation of hydrophobic comonomer MEA shifted the VPTT of poly（DEGMMA-co-MEA） microgels to lower temperatures. The interfacial interaction of poly（DEGMMA-co-MEA） microgels and three model proteins, namely fibrinogen, bovine serum albumin and lysozyme, was investigated by quartz crystal microbalance（QCM）. An injection sequence of ＂microgel-after-protein＂ was then established for the real-time study of the interaction of proteins and the microgels at their swollen and collapsed states by using QCM technique. The results indicated that the interfacial interaction of poly（DEGMMA-co-MEA） microgels and adsorbed protein layers was mainly determined by the electrostatic interaction. Because poly（DEGMMA-co-MEA） microgels were negatively charged in Tris-HCl buffer solution（pH = 7.4）, the microgels did not adsorb on negatively charged fibrinogen and bovine serum albumin layers but strongly adsorbed on positively charged lysozyme layer. Stronger interaction between lysozyme and the microgels at collapsed state（i.e. at 37 ℃） was observed. Furthermore, the incorporation of MEA might weaken the interaction between poly（DEGMMA-co-MEA） microgels and proteins.