Upconversion nanoparticles for differential imaging of plant cells and detection of fluorescent dyes

作     者：吴笑峰 胡盼 胡仕刚 陈增辉 严焕元 唐志军 席在芳 余意 戴港涛 刘云新 WU Xiaofeng;HU Pan;HU Shigang;CHEN Zenghui;YAN Huanyuan;TANG Zhijun;XI Zaifang;YU Yi;DAI Gangtao;LIU Yunxin

作者机构：School of Information and Electrical Engineering Department of Physics and Electrical Science College of Mechanical and Electrical EngineeringHunan University of Science and TechnologyXiangtan 411201China INPAC-Institute for Nanoscale Physics and ChemsitryKU LeuvenCelestijnenlaan 200D B-3001Belgium 

出 版 物：《Journal of Rare Earths》 (稀土学报（英文版）)

年 卷 期：2016年第34卷第2期

页      面：208-220页

核心收录：

学科分类：0710[理学-生物学] 081702[工学-化学工艺] 0709[理学-地质学] 0819[工学-矿业工程] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0708[理学-地球物理学] 071009[理学-细胞生物学] 0703[理学-化学] 

基　　金：supported by the National Natural Science Foundation of China(21301058,61376076,61377024) the Scientific Research Fund of Hunan Provincial Education Department(14B060) Natural Science Foundation of Hunan Province(13JJ4048) 

主　　题：upconversion luminescence fluorescent dye cell imaging detection rare earths 

摘      要：Upconversion NaLuF4 nanoparticles were synthesized by the solvothermal method which could emit multicolor visible light under the excitation of 980 nm near-infrared (NIR) photons. These upconversion nanoparticles (UCNPs) with an acidic ligand could rapidly capture the basic rhodamine-B (RB) in plant cells to generate a close UCNPs@RB system. RB could efficiently absorb the green fluorescence from NaLuF4:18 mol.%Yb3+,2 mol.%Er3+ UCNPs and then emitted red light in the UCNPs@RB system by a robust luminescence resonance energy transfer (LRET) from UCNPs to RB. The detection limit of RB with these upconversion fluo-rescent nanoprobes could reach 0.25μg/cm3 in plant cell even under an ultra low excitation power source of 0.2 W/mm2. This LRET phenomenon was also extended to NaLuF4:18 mol.%Yb3+,0.5 mol.%Tm3+@Sodium fluorescein (SF) system. In addition, the differ-ential imaging could be achieved by successively incubating plant cells with fluorescent dyes and UCNPs. The fluorescent dyes ag-gregated in cell wall while UCNPs with surface modification distributed both in cell wall and cytoplasm, so that UCNPs@Dyes formed in cell walls which could emit multicolor light by LRET which was different from the emission in cytoplasm with only UCNPs.