The discovery of archaea origin phosphomannomutase in algae based on the algal transcriptome

作     者：FENG Yanjing CHI Shan LIU Cui CHEN Shengping YU Jun WANG Xumin LIU Tao 

作者机构：College of Marine Life ScienceOcean University of China Guangdong Province Key Laboratory of Functional Molecules in Oceanic MicroorganismZhong Shan School of MedicineSun Yat-Sen University CAS Key Laboratory of Genome Sciences and InformationBeijing Key Laboratory of Genome and Precision Medicine TechnologiesBeijing Institute of GenomicsChinese Academy of Sciences Beijing Key Laboratory of Functional Genomics for Dao-di HerbsBeijing Institute of GenomicsChineseAcademy of Sciences 

出 版 物：《Acta Oceanologica Sinica》 (海洋学报（英文版）)

年 卷 期：2014年第33卷第2期

页      面：108-113页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：The National High Technology Research and Development Program of China under contract No.2012AA10A406 the National Nat-ural Science Foundation of China under contract Nos 41206116,31140070 and 31271397 Technology Project of Ocean and Fisheries of Guangdong Province under contract No.A201201E03 the Fundamental Research Funds for the Central Universities under contract No.201262003 China Post-doctoral Science Foundation under contract No.2011M501167 the algal transcriptome sequencing was supported by 1KP Project(www.onekp.com) 

主　　题：transcriptomic sequencing Chromista phosphomannomutase conserved motif phylogenetic analysis 

摘      要：Phosphomannomutase （PMM;EC 5.***.2.8） is an enzyme that catalyzes the interconversion reaction between mannose-6-phosphate and mannose-1-phosphate. However, its systematic molecular and functional in-vestigations in algae have not hitherto been reported. In this work, with the accomplishment of the 1 000 Plant Project （OneKP） in which more than 218 species of Chromista, including 19 marine phaeophytes, 22 marine rhodophytes, 171 chlorophytes, 5 cryptophytes, 4 haptophytes, and 5 glaucophytes were sequenced, we used a gene analysis method to analyze the PMM gene sequences in algae and confirm the existence of the PMM gene in the transcriptomic sequencing data of Rhodophyta and Ochrophyta. Our results showed that only one type of PMM with four conserved motifs exists in Chromista which is similar to human PMM. Moreover, the phylogenetic tree revealed that algae PMM possibly originated from archaea.