Effects of penehyclidine hydrochloride in small intestinal damage caused by limb ischemia-reperfusion
Effects of penehyclidine hydrochloride in small intestinal damage caused by limb ischemia-reperfusion作者机构：Department of Anesthesiology First HospitalLanzhou University Lanzhou 730000 Gansu Province China
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2011年第17卷第2期
页 面：254-259页
核心收录：
学科分类：0710[理学-生物学] 090502[农学-动物营养与饲料科学] 1002[医学-临床医学] 07[理学] 0905[农学-畜牧学] 09[农学] 071002[理学-动物学]
基 金：Supported by Lanzhou City Development Plan of Science and Technology No.2009-1-52
主 题：Penehyelidine hydrochloride Post-conditioning Limb ischemia-reperfusion injury Small intestine Protection
摘 要：AIM:To investigate the protective effect of penehyclidine hydrochloride post-conditioning in the damage to the barrier function of the small intestinal mucosa caused by limb ischemia-reperfusion(LIR) injury. METHODS:Male Wistar rats were randomly divided into three groups(36 rats each) :the sham-operation group(group S) ,lower limb ischemia-reperfusion group(group LIR) ,and penehyclidine hydrochloride postconditioning group(group PHC) .Each group was divided into subgroups(n=6 in each group) according to ischemic-reperfusion time,*** 0 h(T1) ,1 h(T2) ,3 h(T3) ,6 h(T4) ,12 h(T5) ,and 24 h(T6) .Bilateral hind-limb ischemia was induced by rubber band application proximal to the level of the greater trochanter for 3 *** group PHC,0.15 mg/kg of penehyclidine hydrochloride was injected into the tail vein immediately after 3 h of bilateral hind-limb *** designated rats were sacrificed at different time-points of reperfusion;diamine oxidase(DAO) ,superoxide dismutase(SOD) activity,myeloperoxidase(MPO) of small intestinal tissue,plasma endotoxin,DAO,tumor necrosis factor-α(TNF-α) ,and interleukin(IL) -10 in serum were detected in the rats. RESULTS:The pathological changes in the small intestine were observed under light *** levels of MPO,endotoxin,serum DAO,and IL-10 at T1-T6,and TNF-αlevel at T1-T4 increased in groups LIR and PHC(P0.05) compared with those in group S,but tissue DAO and SOD activity at T1-T6 decreased(P0.05) .In group PHC,the tissue DAO and SOD activity at T2-T6,and IL-10 at T2-T5 increased to higher levels than those in group LIR(P0.05) ;however,the levels of MPO,endotoxin,and DAO in the blood at T2-T6,and TNF-αat T2 and T4 decreased(P0.05) . CONCLUSION:Penehyclidine hydrochloride post-conditioning may reduce the permeability of the small intestines after *** protection mechanisms may be related to inhibiting oxygen free radicals and inflammatory cytokines for organ damage.
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