Highly sensitive detection of melamine based on reversed phase liquid chromatography mass spectrometry

作     者：WU QingQing FAN KeXin SHA Wei RUAN HongQiang ZENG Rong SHIEH ChiaHui 

作者机构：Key Laboratory of Systems Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China Shanghai KeyTag Bioscience Inc. Shanghai 200233 China 

出 版 物：《Chinese Science Bulletin》 (CHINESE SCIENCE BULLETIN)

年 卷 期：2009年第54卷第5期

页      面：732-737页

核心收录：

学科分类：07[理学] 070302[理学-分析化学] 0703[理学-化学] 

基　　金：Supported by the National Natural Science Foundation of China (Grant No. 30425021) National High Technology Research and Development Program of China (Grant No. 2007AA02Z334) 

主　　题：反相流相色谱 质谱分析法 离子对 多反应监测模式 三聚氰胺 

摘      要：In this work, we developed a highly sensitive method to detect melamine based on reversed phase liquid chromatography mass spectrometry. A mass spectrometry compatible ion pair, heptafluorobu-tyric acid(HFBA), was used to separate melamine by reversed phase liquid chromatography prior to electrospray mass spectrometry. The incorporation of isotope internal standard and multiple reaction monitoring improved the accuracy and linearity of quantification. Based on this strategy, the method limit of quantification was 0.1 ng/g. The limits of quantification were 8 ng/g for liquid milk and 15 ng/g for dry milk powder. This method provided a reproducible and stable approach to sensitive detection and quantification of melamine.